GP_LIG_1_read1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	GP_LIG_1_read1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4543708
Sequences flagged as poor quality	0
Sequence length	150
%GC	68

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per tile quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTATTGCATCTCGTAT	210407	4.630733312968175	TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTATTGCATCGCGTAT	127558	2.8073546979691475	TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTATTGCATCTCGTTT	13864	0.30512524132272584	TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTATTGCATCGCGTTT	8992	0.19790004111179682	TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTATTGCATCTCGGAT	7626	0.16783648949272267	TruSeq Adapter, Index 8 (97% over 36bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACACTATTGCATCTCGTA	7345	0.16165211320797904	TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTATTGCATCGCGGAT	5780	0.12720887873956688	TruSeq Adapter, Index 8 (97% over 36bp)

Adapter Content

Adapter graph

Basic Statistics

Measure	Value
Filename	GP_LIG_1_read2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4543708
Sequences flagged as poor quality	0
Sequence length	150
%GC	67

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per tile quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	27557	0.6064870365789351	No Hit

Adapter Content

Adapter graph

Basic Statistics

Measure	Value
Filename	GP_LIG_2_read1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13141442
Sequences flagged as poor quality	0
Sequence length	150
%GC	69

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per tile quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACGATTCATCTCGTAT	1602183	12.191835568729823	TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACGATTCATCGCGTAT	1059470	8.0620528553868	TruSeq Adapter, Index 7 (97% over 35bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACTACGATTCATCTCGTA	46693	0.3553110838216993	TruSeq Adapter, Index 10 (97% over 35bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACTACGATTCATCGCGTA	30217	0.22993671470756405	TruSeq Adapter, Index 10 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTACGATTCATCTCGTATG	13148	0.10004990319935969	TruSeq Adapter, Index 7 (97% over 34bp)

Adapter Content

Adapter graph

Basic Statistics

Measure	Value
Filename	GP_LIG_2_read2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13141442
Sequences flagged as poor quality	0
Sequence length	150
%GC	71

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per tile quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	196252	1.4933825374719152	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG	19451	0.14801267623446498	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT	13595	0.10345135640365799	No Hit

Adapter Content

Adapter graph

Basic Statistics

Measure	Value
Filename	GP_LIG_3_read1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5019203
Sequences flagged as poor quality	0
Sequence length	150
%GC	70

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per tile quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCAGTGGATCTCGTAT	114381	2.278867780402586	TruSeq Adapter, Index 11 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCAGTGGATCGCGTAT	60310	1.201585191911943	TruSeq Adapter, Index 11 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCAGTGGATCTCGTTT	24416	0.4864517334724258	TruSeq Adapter, Index 11 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCAGTGGATCTCGGAT	18588	0.3703376811019598	TruSeq Adapter, Index 11 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCAGTGGATCGCGTTT	13800	0.2749440498820231	TruSeq Adapter, Index 11 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCAGTGGATCGCGGAT	11072	0.22059279132563475	TruSeq Adapter, Index 11 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCAGTGGATCTCGGTT	8298	0.165325052602973	TruSeq Adapter, Index 11 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCAGTGGATCGCGGTT	5694	0.11344430579914778	TruSeq Adapter, Index 11 (97% over 36bp)

Adapter Content

Adapter graph

Basic Statistics

Measure	Value
Filename	GP_LIG_3_read2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5019203
Sequences flagged as poor quality	0
Sequence length	150
%GC	66

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per tile quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	19990	0.39827040269142333	No Hit

Adapter Content

Adapter graph

Basic Statistics

Measure	Value
Filename	GP_LIG_4_read1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16087830
Sequences flagged as poor quality	0
Sequence length	150
%GC	71

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per tile quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCGAGGAATCTCGGTT	184562	1.147215006623019	TruSeq Adapter, Index 11 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCGAGGAATCGCGGTT	95036	0.5907322491597686	TruSeq Adapter, Index 11 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCGAGGAATCTCGGGT	83412	0.5184788750254075	TruSeq Adapter, Index 11 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCGAGGAATCGCGGGT	58967	0.36653171993985517	TruSeq Adapter, Index 11 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCGAGGAATCTCGTTT	47748	0.29679577668336876	TruSeq Adapter, Index 11 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCGAGGAATCGCGTTT	22862	0.14210741908635285	TruSeq Adapter, Index 11 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCGAGGAATCTCGGAT	20611	0.1281154761083378	TruSeq Adapter, Index 11 (97% over 36bp)

Adapter Content

Adapter graph

Basic Statistics

Measure	Value
Filename	GP_LIG_4_read2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16087830
Sequences flagged as poor quality	0
Sequence length	150
%GC	66

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per tile quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	52969	0.3292488794324654	No Hit

Adapter Content

Adapter graph

Basic Statistics

Measure	Value
Filename	GP_LIG_5_read1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5872884
Sequences flagged as poor quality	0
Sequence length	150
%GC	71

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per tile quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGCCACAATCTCGGTT	81707	1.3912585366916834	TruSeq Adapter, Index 15 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGCCACAATCGCGGTT	39059	0.6650735822468143	TruSeq Adapter, Index 15 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGCCACAATCTCGGGT	35571	0.6056819783942609	TruSeq Adapter, Index 15 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGCCACAATCTCGTTT	25845	0.4400733949453114	TruSeq Adapter, Index 15 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGCCACAATCGCGGGT	22646	0.3856027123982016	TruSeq Adapter, Index 15 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGCCACAATCGCGTTT	11889	0.20243886989765167	TruSeq Adapter, Index 15 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGCCACAATCTCGGAT	11377	0.19372083630461628	TruSeq Adapter, Index 15 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGCCACAATCTCGTGT	8647	0.14723600874800183	TruSeq Adapter, Index 15 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGCCACAATCTCGTAT	6882	0.11718263122513572	TruSeq Adapter, Index 15 (97% over 36bp)

Adapter Content

Adapter graph

Basic Statistics

Measure	Value
Filename	GP_LIG_5_read2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5872884
Sequences flagged as poor quality	0
Sequence length	150
%GC	67

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per tile quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	28761	0.48972532064314567	No Hit

Adapter Content

Adapter graph

Basic Statistics

Measure	Value
Filename	GP_LIG_6_read1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17287587
Sequences flagged as poor quality	0
Sequence length	150
%GC	70

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per tile quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCGGTCATCTCGTAT	835587	4.833450729705655	TruSeq Adapter, Index 27 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCGGTCATCGCGTAT	623657	3.607542220901043	TruSeq Adapter, Index 27 (97% over 37bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCGGTCATCTCGTA	28495	0.16482925002778004	TruSeq Adapter, Index 12 (97% over 36bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCGGTCATCGCGTA	22868	0.1322798838264704	TruSeq Adapter, Index 12 (97% over 36bp)

Adapter Content

Adapter graph

Basic Statistics

Measure	Value
Filename	GP_LIG_6_read2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17287587
Sequences flagged as poor quality	0
Sequence length	150
%GC	67

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per tile quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	117003	0.6768035353921864	No Hit

Adapter Content

Adapter graph

Basic Statistics

Measure	Value
Filename	GP_LIG_7_read1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5939440
Sequences flagged as poor quality	0
Sequence length	150
%GC	69

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per tile quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAGGTATCTCGTAT	104148	1.7534986463370286	TruSeq Adapter, Index 25 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAGGTATCGCGTAT	71143	1.197806527214687	TruSeq Adapter, Index 25 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAGGTATCTCGTTT	6119	0.10302318063655833	TruSeq Adapter, Index 25 (97% over 38bp)

Adapter Content

Adapter graph

Basic Statistics

Measure	Value
Filename	GP_LIG_7_read2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5939440
Sequences flagged as poor quality	0
Sequence length	150
%GC	66

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per tile quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	18707	0.31496235335317807	No Hit

Adapter Content

Adapter graph

Basic Statistics

Measure	Value
Filename	GP_LIG_8_read1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17498828
Sequences flagged as poor quality	0
Sequence length	150
%GC	68

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per tile quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAATCAGATCTCGTAT	547199	3.1270608523039374	TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAATCAGATCGCGTAT	214485	1.2257106590224214	TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAATCAGATCTCGGAT	64042	0.36597879583706977	TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAATCAGATCTCGTTT	58190	0.33253655616250416	TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAATCAGATCGCGGAT	30023	0.17157149038781339	TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAATCAGATCGCGTTT	24712	0.14122088633593063	TruSeq Adapter, Index 2 (97% over 36bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAATCAGATCTCGTA	17714	0.10122963663623645	TruSeq Adapter, Index 13 (97% over 35bp)

Adapter Content

Adapter graph

Basic Statistics

Measure	Value
Filename	GP_LIG_8_read2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17498828
Sequences flagged as poor quality	0
Sequence length	150
%GC	67

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per tile quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	78941	0.45112164083217454	No Hit

Adapter Content

Adapter graph

Basic Statistics

Measure	Value
Filename	GP_PCR_1_read1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4415432
Sequences flagged as poor quality	0
Sequence length	150
%GC	54

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per tile quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

No overrepresented sequences

Adapter Content

Adapter graph

Basic Statistics

Measure	Value
Filename	GP_PCR_1_read2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4415432
Sequences flagged as poor quality	0
Sequence length	150
%GC	54

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
ATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCAC	4252468	96.30921730874805	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	4633	0.1049274453779381	No Hit

Adapter Content

Adapter graph

Basic Statistics

Measure	Value
Filename	GP_PCR_2_read1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12697763
Sequences flagged as poor quality	0
Sequence length	150
%GC	54

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per tile quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

No overrepresented sequences

Adapter Content

Adapter graph

Basic Statistics

Measure	Value
Filename	GP_PCR_2_read2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12697763
Sequences flagged as poor quality	0
Sequence length	150
%GC	54

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per tile quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
ATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCAC	12274734	96.66847617174773	No Hit
ATGTGCTGCAAGGCGAGTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCAC	19517	0.15370423908526248	No Hit

Adapter Content

Adapter graph

Basic Statistics

Measure	Value
Filename	GP_PCR_3_read1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5434358
Sequences flagged as poor quality	0
Sequence length	150
%GC	54

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per tile quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

No overrepresented sequences

Adapter Content

Adapter graph

Basic Statistics

Measure	Value
Filename	GP_PCR_3_read2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5434358
Sequences flagged as poor quality	0
Sequence length	150
%GC	54

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per tile quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
ATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCAC	5236980	96.36796103605982	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	5779	0.10634190828061015	No Hit
ATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTCCCAGTCACG	5615	0.10332407250313652	No Hit

Adapter Content

Basic Statistics

Measure	Value
Filename	GP_PCR_4_read1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14595572
Sequences flagged as poor quality	0
Sequence length	150
%GC	54

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per tile quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

No overrepresented sequences

Adapter Content

Adapter graph

Basic Statistics

Measure	Value
Filename	GP_PCR_4_read2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14595572
Sequences flagged as poor quality	0
Sequence length	150
%GC	54

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per tile quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
ATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCAC	13971050	95.72115433365681	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	15401	0.105518303770486	No Hit

Adapter Content

Adapter graph

Basic Statistics

Measure	Value
Filename	GP_PCR_5_read1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4202038
Sequences flagged as poor quality	0
Sequence length	150
%GC	54

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per tile quality graph

Per sequence quality scores

$Per Sequence quality graph$

Per base sequence content

Per sequence GC content

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

No overrepresented sequences

Adapter Content

Adapter graph

Basic Statistics

Measure	Value
Filename	GP_PCR_5_read2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4202038
Sequences flagged as poor quality	0
Sequence length	150
%GC	54

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per tile quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
ATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCAC	4044714	96.25600720412334	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	4620	0.10994664969712316	No Hit
ATGTGCTGCAAGGCGAGTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCAC	4531	0.10782862982200543	No Hit

Adapter Content

Adapter graph

Basic Statistics

Measure	Value
Filename	GP_PCR_6_read1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12608135
Sequences flagged as poor quality	0
Sequence length	150
%GC	54

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per tile quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

No overrepresented sequences

Adapter Content

Adapter graph

Basic Statistics

Measure	Value
Filename	GP_PCR_6_read2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12608135
Sequences flagged as poor quality	0
Sequence length	150
%GC	54

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per tile quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
ATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCAC	12149598	96.36316552765336	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	13280	0.10532882143155986	No Hit

Adapter Content

Adapter graph

Basic Statistics

Measure	Value
Filename	GP_PCR_7_read1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5542451
Sequences flagged as poor quality	0
Sequence length	150
%GC	54

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per tile quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

No overrepresented sequences

Adapter Content

Adapter graph

Basic Statistics

Measure	Value
Filename	GP_PCR_7_read2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5542451
Sequences flagged as poor quality	0
Sequence length	150
%GC	54

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per tile quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
ATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCAC	5334900	96.25524880598854	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	6210	0.11204429231760461	No Hit
ATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTCCCAGTCACG	5733	0.10343799160335383	No Hit

Adapter Content

Adapter graph

Basic Statistics

Measure	Value
Filename	GP_PCR_8_read1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14906452
Sequences flagged as poor quality	0
Sequence length	150
%GC	54

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per tile quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

No overrepresented sequences

Adapter Content

Adapter graph

Basic Statistics

Measure	Value
Filename	GP_PCR_8_read2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14906452
Sequences flagged as poor quality	0
Sequence length	150
%GC	54

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per tile quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
ATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCAC	14269165	95.72475730643349	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	16285	0.1092479954317768	No Hit

Adapter Content

Adapter graph

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content